Ethanol Neogenesis in Stored Blood by Komal Singh in Peer Reviewed Journal of Forensic & Genetic Sciences (PRJFGS) in Lupine Publishers
During
the investigation of accidental cases, blood samples obtained from accidental
victim are submitted to forensic science laboratory for toxicological analysis.
All through, ethanol analysis is performed on almost all cases of blood
samples. The main problem is determining alcohol concentration in these cases
is changes in Blood Alcohol Concentration (BAC) that can occur due to neo
formation of ethanol. Blood samples stored under in sufficient conditions or
for longer times and body fluids have undergone petrifaction often contains
certain amount of volatile compounds. In the light of possibility that, neo
formation of ethanol in improperly stored blood might give rise to an incorrect
estimation of BAC. With this in mind, to study the effect of preservative and
temperature on the formation and concentration of ethanol in blood samples, we
estimated the ethanol neo genesis in stored and normal blood in different time
period (0, 7, 14, 21, 28, 35 and 42 days). Head Space-Gas Chromatography
instrument used to measure the level of ethanol in blood sample. We found that
neo formation of ethanol was observed in normal blood sample. There was no any
neo formation observed in blood sample with preservative and blood stored at 4
°C. Our study is supporting the studies which suggest that storage condition
will affect the neo formation of ethanol in blood samples.
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